Human Myeloproliferative Leukemia Virus Oncogene (MPL) ELISA Kit

SKU: orb779453

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloproliferative Leukemia Virus Oncogene(MPL).

Research Area

Cancer Biology, Hematology, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MPL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.108 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD110 ELISA Kit, C-MPL ELISA Kit, MPLV ELISA Kit, TPOR ELISA Kit, Thrombopoietin Receptor ELISA Kit, Myeloproliferative leukemia protein ELISA Kit, Proto-oncogene c-Mpl ELISA Kit