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Human Myeloperoxidase (MPO) ELISA Kit

Human Myeloperoxidase (MPO) ELISA Kit

Catalog Number: orb2668117

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2668117
CategoryAssays and Kits
DescriptionHuman Myeloperoxidase (MPO) ELISA Kit
UniProt IDP05164
Application notesstandard: 150 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MPO in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range2.34-150 ng/mL
Sensitivity0.65 ng/mL
Sample TypesSerum, plasma and other biological fluids.
Alternative namesMpo; Myeloperoxidase ; MPO
NoteFor research use only
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