Human Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1) ELISA Kit

SKU: orb777797

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Cell Leukemia Sequence 1, Bcl2 Related(MCL1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MCL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MCL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MCL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MCL1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.123 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

EAT ELISA Kit, MCL1L ELISA Kit, MCL1S ELISA Kit, BCL2L3 ELISA Kit, TM ELISA Kit, Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 ELISA Kit, Bcl-2-like protein 3 ELISA Kit, Bcl-2-related protein EAT/mcl1 ELISA Kit, mcl1/EAT ELISA Kit