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Human Myelin Basic Protein (MBP) ELISA Kit

Catalog Number: orb1807363

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1807363
CategoryAssays and Kits
DescriptionHuman Myelin Basic Protein (MBP) ELISA Kit
TargetMBP
ReactivityHuman
UniProt IDP02686
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MBP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MBP. You can calculate the concentration of Human MBP in the samples by comparing the OD of the samples to the standard curve.
Assay TypeSandwich
Assay Time3.5H
Standard1000 pg/mL
Range15.63-1000 pg/mL
Sensitivity5.47 pg/mL
Sample Typesserum, plasma, Tissue homogenate and Other biological samples
Alternative namesMyelin_MBP
NoteFor research use only
Human Myelin Basic Protein (MBP) ELISA Kit

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