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| Catalog Number | orb778744 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone H2B(H2B). |
| Reactivity | Human, Mouse, Rat |
| Concentration | 200 ng/mL |
| Tested applications | ELISA |
| Application notes | standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human,Rat,Mouse H2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human,Rat,Mouse H2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human,Rat,Mouse H2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human,Rat,Mouse H2B in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 3.13-200 ng/mL |
| Sensitivity | 1.15 ng/mL |
| Sample Types | tissue homogenates, cell lysates and other biological fluids |
| Alternative names | HIST1H2BA, testis, bA317E16.3, H2B, H2B histone fa Read more... |
| Note | For research use only |
