Human Monokine Induced by Interferon Gamma (MIg) EasyStep ELISA Kit

• Catalog Number: orb1950033
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIg, and the Human MIg standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MIg. After TMB substrate solution is added, only those wells that contain Human MIg and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIg in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Human
• Concentration: 5000 pg/mL
• UniProt ID: Q07325
• Application notes: standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIg, and the Human MIg standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MIg. After TMB substrate solution is added, only those wells that contain Human MIg and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIg in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 78.2-5000 pg/mL
• Sensitivity: 15.7 pg/mL
• Sample Types: Serum, plasma
• Alternative names: CXCL9; CMK; Humig; MI-G; SCYB9; Crg-10; Chemokine
• Research Area: Immunology & Inflammation, Infectious Disease & Vi
• Note: For research use only
• Expiration Date: Please enquire.