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Catalog Number | orb392149 |
---|---|
Category | Proteins |
Description | Recombinant human MMP9 protein is produced by our mammalian expression system and the target gene encoding Ala19-Asp707 is expressed with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Human |
Form/Appearance | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 2mM CaCl2, 150mM NaCl, 0.05% Brij35, pH 7.5. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | MMP9 |
Entrez | 4318 |
UniProt ID | P14780 |
Protein Sequence | AAPRQRQSTL VLFPGDLRTN LTDRQLAEEY LYRYGYTRVA EMRGESKSLG PALLLLQKQL SLPETGELDS ATLKAMRTPR CGVPDLGRFQ TFEGDLKWHH HNITYWIQNY SEDLPRAVID DAFARAFALW SAVTPLTFTR VYSRDADIVI QFGVAEHGDG YPFDGKDGLL AHAFPPGPGI QGDAHFDDDE LWSLGKGVVV PTRFGNADGA ACHFPFIFEG RSYSACTTDG RSDGLPWCST TANYDTDDRF GFCPSERLYT RDGNADGKPC QFPFIFQGQS YSACTTDGRS DGYRWCATTA NYDRDKLFGF CPTRADSTVM GGNSAGELCV FPFTFLGKEY STCTSEGRGD GRLWCATTSN FDSDKKWGFC PDQGYSLFLV AAHEFGHALG LDHSSVPEAL MYPMYRFTEG PPLHKDDVNG IRHLYGPRPE PEPRPPTTTT PQPTAPPTVC PTGPPTVHPS ERPTAGPTGP PSAGPTGPPT AGPSTATTVP LSPVDDACNV NIFDAIAEIG NQLYLFKDGK YWRFSEGRGS RPQGPFLIAD KWPALPRKLD SVFEEPLSKK LFFFSGRQVW VYTGASVLGP RRLDKLGLGA DVAQVTGALR SGRGKMLLFS GRRLWRFDVK AQMVDPRSAS EVDRMFPGVP LDTHDVFQYR EKAYFCQDRF YWRVSSRSEL NQVDQVGYVT YDILQCPEDV DHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Buffer/Preservatives | Liquid in a 0.2 μM filtered solution of 20mM Tris-HCl, 2mM CaCl2, 150mM NaCl, 0.05% Brij35, pH 7.5. |
Alternative names | CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa g Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 48.5 kDa after removal of the signal peptide. The apparent molecular mass of MMP9-His is approximately 55-70 kDa due to glycosylation. | |
Mammalian |
Greater than 95% as determined by SDS-PAGE. | |
46.8 kDa | |
Mammalian cell |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 77.2 kDa after removal of the signal peptide. The apparent molecular mass of MMP9(20-707)-His is approximately 70-100 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by SDS-PAGE and HPLC. | |
20.5 kDa | |
E.Coli |