Human MMP9 protein

SKU: orb392149

Description

Recombinant human MMP9 protein is produced by our mammalian expression system and the target gene encoding Ala19-Asp707 is expressed with a 6His tag at the C-terminus.

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, MS, SDS-PAGE, WB
• Application Notes:
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

• Source: Human cells
• Target: MMP9
• Reactivity: Human
• Endotoxins: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Protein Sequence: AAPRQRQSTL VLFPGDLRTN LTDRQLAEEY LYRYGYTRVA EMRGESKSLG PALLLLQKQL SLPETGELDS ATLKAMRTPR CGVPDLGRFQ TFEGDLKWHH HNITYWIQNY SEDLPRAVID DAFARAFALW SAVTPLTFTR VYSRDADIVI QFGVAEHGDG YPFDGKDGLL AHAFPPGPGI QGDAHFDDDE LWSLGKGVVV PTRFGNADGA ACHFPFIFEG RSYSACTTDG RSDGLPWCST TANYDTDDRF GFCPSERLYT RDGNADGKPC QFPFIFQGQS YSACTTDGRS DGYRWCATTA NYDRDKLFGF CPTRADSTVM GGNSAGELCV FPFTFLGKEY STCTSEGRGD GRLWCATTSN FDSDKKWGFC PDQGYSLFLV AAHEFGHALG LDHSSVPEAL MYPMYRFTEG PPLHKDDVNG IRHLYGPRPE PEPRPPTTTT PQPTAPPTVC PTGPPTVHPS ERPTAGPTGP PSAGPTGPPT AGPSTATTVP LSPVDDACNV NIFDAIAEIG NQLYLFKDGK YWRFSEGRGS RPQGPFLIAD KWPALPRKLD SVFEEPLSKK LFFFSGRQVW VYTGASVLGP RRLDKLGLGA DVAQVTGALR SGRGKMLLFS GRRLWRFDVK AQMVDPRSAS EVDRMFPGVP LDTHDVFQYR EKAYFCQDRF YWRVSSRSEL NQVDQVGYVT YDILQCPEDV DHHHHHH

Storage & Handling

• Storage: Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.2 μM filtered solution of 20mM Tris-HCl, 2mM CaCl2, 150mM NaCl, 0.05% Brij35, pH 7.5.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CLG4B; Matrix metalloproteinase-9; MMP-9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

Quick Database Links

Gene Symbol

MMP9

UniProt

P14780

NCBI Gene ID

4318