Human Matrix Metalloproteinase 9 (MMP9) EasyStep ELISA Kit

SKU: orb1950019

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9, and the Human MMP9 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP9. After TMB substrate solution is added, only those wells that contain Human MMP9 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area

Cancer Biology, Cardiovascular Research, Gastroenterology & Hepatology, Infectious Disease & Virology, Protein Biochemistry

Images & Validation

• Application Notes:
  standard: 80 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP9, and the Human MMP9 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human MMP9. After TMB substrate solution is added, only those wells that contain Human MMP9 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma
• Assay Type: Sandwich
• Assay Time: 1.5h
• Range: 1.25-80 ng/mL
• Sensitivity: 1.25 ng/mL
• Concentration: 80 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase

Quick Database Links

UniProt

P14780