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Human MMP13 Protein, His Tag
Description
Research Area
Images & Validation
−Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | MMP13 |
| Reactivity | Human |
| Tag | C-6×His Tag |
| Molecular Weight | The protein has a predicted molecular mass of 52.5 kDa after removal of the signal peptide. The apparent molecular mass of MMP13-His is approximately 55-70 kDa due to glycosylation. |
| Expression Region | MMP13(Leu20-Cys471) 6×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Recombinant Human MMP13 Protein, N-His [orb2968511]
ELISA, SDS-PAGE, WB
>90% as determined by SDS-PAGE.
53.97 kDa
1 mg, 50 μg, 100 μgRecombinant Human MMP13 Protein, N-His [orb2821293]
>90% as determined by SDS-PAGE.
53.99 kDa
20 μg, 50 μg, 100 μgHuman MMP13 Protein, His Tag (Pro-form, HPLC verified) [orb3158090]
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95%
30.5 kDa
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Human BTLA Protein, His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human BTLA, His tagged protein (orb757467) can bind Anti-BTLA Neutralizing antibody orb1173646 in a linear range of 16-80 ng/ml.

Flow cytometry analysis with 15 μg/mL Human BTLA Protein, His tag (orb757467) on Expi293 cells transfected with human HVEM (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Quick Database Links
UniProt Details
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Protocol Information
Human MMP13 Protein, His Tag (orb1173677)
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