Human Mitogen Activated Protein Kinase 9 (MAPK9) ELISA Kit

SKU: orb779399

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 9(MAPK9).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAPK9. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAPK9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAPK9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAPK9 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.04 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

JNK-55 ELISA Kit, JNK2 ELISA Kit, JNK2A ELISA Kit, JNK2ALPHA ELISA Kit, JNK2B ELISA Kit, JNK2BETA ELISA Kit, PRKM9 ELISA Kit, p54a ELISA Kit, SAPK ELISA Kit, Jun Kinase ELISA Kit, Stress-activated protein kinase 1a ELISA Kit, c-Jun N-terminal kinase 2 ELISA Kit

Quick Database Links

UniProt

P45984