Human Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B) ELISA Kit

• Catalog Number: orb782414
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B).
• Reactivity: Human
• Concentration: 10 ng/mL
• UniProt ID: A6NCE7
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP1LC3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Alternative names: LC3B ELISA Kit, MAP1LC3B ELISA Kit, MAP1ALC3 ELISA
• Research Area: Metabolism Research
• Note: For research use only