Human Microseminoprotein Beta (MSMb) ELISA Kit

SKU: orb777365

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microseminoprotein Beta(MSMb).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MSMb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MSMb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MSMb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MSMb in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, seminal plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.063 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MSM-B ELISA Kit, IGBF ELISA Kit, MSP ELISA Kit, MSPB ELISA Kit, PN44 ELISA Kit, PRPS ELISA Kit, PSP ELISA Kit, PSP57 ELISA Kit, PSP94 ELISA Kit, Immunoglobulin-binding factor ELISA Kit, Prostate secreted seminal plasma protein ELISA Kit, plasma beta-inhibin ELISA Kit

Quick Database Links

UniProt

P08118