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Human Microfibrillar Associated Protein 5 (MFAP5) ELISA Kit

Human Microfibrillar Associated Protein 5 (MFAP5) ELISA Kit

Catalog Number: orb2667047

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb2667047
CategoryAssays and Kits
DescriptionHuman Microfibrillar Associated Protein 5 (MFAP5) ELISA Kit
Range0.16-10 ng/mL
Assay Time3.5h
UniProt IDQ13361
Sensitivity0.061 ng/mL
Alternative namesMAGP2; MP25; Microfibril-associated glycoprotein 2
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NoteFor research use only
Application notesstandard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MFAP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MFAP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MFAP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MFAP5 in the samples is then determined by comparing the OD of the samples to the standard curve
Sample Typestissue homogenates, cell lysates, cell culture supernates and other biological fluids
Expiration DatePlease enquire.
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