Human Meteorin Like Protein (METRNL) ELISA Kit

SKU: orb781274

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Meteorin Like Protein(METRNL).

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human METRNL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human METRNL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human METRNL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human METRNL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.66 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Subfatin ELISA Kit, Glial Cell Differentiation Regulator Like ELISA Kit

Standard curve for orb781274 ELISA kit.

Quick Database Links

UniProt

Q641Q3

Samet Can Demirbaş et al. Determination of Serum Meteorin-Like Protein (Metrnl/Subfatin) Levels in Obese Children and Adolescents researchsquare.com, (2026)

PubMed:

Applications

ELISA

Reactivity

Human