Human metapneumovirus Post-fusion glycoprotein F0, His Tag

SKU: orb1743006

Description

Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. The RSV fusion glycoprotein (RSV F) is the principal target of RSV neutralizing antibodies in human sera. The RSV F is a type I viral fusion protein synthesized as inactive, single-chain polypeptides that assemble into trimers. RSV F fuses the viral and host cell membranes by irreversible protein refolding from the labile prefusion conformation to the stable post-fusion conformation. Both states exhibit epitopes targeted by neutralizing antibodies, and post-fusion RSV F is being developed as a vaccine candidate.

Research Area

Infectious Disease & Virology

Images & Validation

• Application Notes:
  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 56.6 kDa. The protein migrates as 51-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Key Properties

• Expression System: HEK293
• Target: Postfusion glycoprotein F0/post-F protein (HMPV)
• Reactivity: Virus
• Tag: C-10×His
• Molecular Weight: 56.6 kDa
• Expression Region: Leu 19- Thr 489
• Purity: 90%
• Endotoxins: 1.0 EU per μg
• Conjugation: Unconjugated

Storage & Handling

• Storage: -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
• Form/Appearance: Powder
• Buffer/Preservatives: 0.1 M Sodium citrate, 0.5 M Arginine, pH 5.5
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Postfusion glycoprotein F0/post-F protein (HMPV)

UniProt

Q6WB98