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Catalog Number | orb624114 |
---|---|
Category | Proteins |
Description | Recombinant Human Mesothelin protein |
Tag | N-terminal hFc-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 92% as determined by SDS-PAGE. |
MW | 59.6 kDa |
UniProt ID | Q13421 |
Protein Sequence | EVEKTACPSGKKAREIDESLIFYKKWELEACVDAALLATQMDRVNAIPFTYEQLDVLKHKLDELYPQGYPESVIQHLGYLFLKMSPEDIRKWNVTSLETLKALLEVNKGHEMSPQVATLIDRFVKGRGQLDKDTLDTLTAFYPGYLCSLSPEELSSVPPSSIWAVRPQDLDTCDPRQLDVLYPKARLAFQNMNGSEYFVKIQSFLGGAPTEDLKALSQQNVSMDLATFMKLRTDAVLPLTVAEVQKLLGPHVEGLKAEERHRPVRDWILRQRQDDLDTLGLGLQG |
Protein Length | Partial of isoform 3 |
Source | Mammalian cell |
Expression System | Expression Region: 296-580aa. Protein Length: Partial of isoform 3 |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized MSLN at 2 μg/ml can bind Anti-MSLN rabbit monoclonal antibody, the EC50 of the MSLN protein is 2.657-3.177 ng/ml. |
Expression Region | 296-580aa |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4 |
Alternative names | CAK1 antigen (Pre-pro-megakaryocyte-potentiating f Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
95% | |
27.5 kDa | |
Human Mesothelin (37-286), His Tag (orb257696) is expressed from human 293 cells (HEK293). It contains AA Leu 37 - Arg 286 (Accession # AAH09272). |
Greater than 90% as determined by SDS-PAGE. | |
36.7 kDa | |
Mammalian cell |
Greater than 85% as determined by SDS-PAGE. | |
61.2 kDa | |
Mammalian cell |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
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