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Catalog Number | orb257696 |
---|---|
Category | Proteins |
Description | Human Mesothelin (37-286), His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 37 - Arg 286 (Accession # AAH09272). |
Target | Mesothelin |
Conjugation | Unconjugated |
Tag | C-6×His |
Reactivity | Human |
Form/Appearance | Powder |
Buffer/Preservatives | PBS, pH 7.4 |
Purity | 95% |
Protein Sequence | AAH09272 |
UniProt ID | Q13421 |
MW | 27.5 kDa |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.5 kDa. The protein migrates as 32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expression System | HEK293 |
Endotoxins | 1.0 EU per μg |
Biological Origin | Human |
Expression Region | Leu 37 - Arg 286 |
Storage | -20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower |
Note | For research use only |
NCBI | AAH09272 |
SDS-PAGE analysis of Human Mesothelin protein
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 59.3 kDa after removal of the signal peptide. | |
Mammalian |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 27.6 kDa after removal of the signal peptide. The apparent molecular mass of Mesothelin(37-286)-His is approximately 25-35 kDa due to glycosylation. | |
Mammalian |
> 95% as determined by SDS-PAGE |
> 90%-95% as determined by SDS-PAGE |