Human Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) ELISA Kit

SKU: orb778156

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Spanning 4 Domains Subfamily A, Member 1(MS4A1).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 80 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MS4A1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MS4A1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MS4A1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MS4A1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.25-80 ng/mL
• Sensitivity: 0.47 ng/mL
• Concentration: 80 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD20 ELISA Kit, MS4-A1 ELISA Kit, B1 ELISA Kit, Bp35 ELISA Kit, LEU16 ELISA Kit, MS4-A2 ELISA Kit, S7 ELISA Kit, B-lymphocyte antigen CD20 ELISA Kit, B-lymphocyte surface antigen B1 ELISA Kit

Quick Database Links

UniProt

P11836