Human Melanoma Derived Leucine Zipper Extra Nuclear Factor (MLZE) ELISA Kit

SKU: orb775997

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Melanoma Derived Leucine Zipper Extra Nuclear Factor(MLZE).

Research Area

Cancer Biology, Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MLZE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MLZE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MLZE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MLZE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.068 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

GSDMC ELISA Kit, Gasdermin C ELISA Kit, Melanoma-Derived Leucine Zipper,Extranuclear Factor ELISA Kit

Quick Database Links

UniProt

Q9BYG8