Human Matrix Metalloproteinase 1 (MMP1) ELISA Kit

SKU: orb776047

Description

The Human Matrix Metalloproteinase 1 (MMP1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Matrix Metalloproteinase 1 (MMP1) in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. The kit has a detection range of 0.16-10 ng/mL and a sensitivity of 0.062 ng/mL.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP1 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.062 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CLGN ELISA Kit, CLG1 ELISA Kit, Collagenase ELISA Kit, Interstitial Collagenase ELISA Kit, Vertebrate Collagenase ELISA Kit, Fibroblast Collagenase ELISA Kit

Quick Database Links

UniProt

P03956