Human Matrix Metalloproteinase 23B (MMP23B) ELISA Kit

SKU: orb775802

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 23B(MMP23B).

Research Area

Cancer Biology, Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 5000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MMP23B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP23B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MMP23B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MMP23B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 78.13-5000 pg/mL
• Sensitivity: 30 pg/mL
• Concentration: 5000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MMP22 ELISA Kit, MMP23-B ELISA Kit, MIFR ELISA Kit, MIFR-1 ELISA Kit, Matrix Metalloproteinase 22 ELISA Kit, Femalysin ELISA Kit, Matrix Metalloproteinase In The Female Reproductive Tract ELISA Kit