Human Matrix Extracellular Phosphoglycoprotein (MEPE) ELISA Kit

SKU: orb775681

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Extracellular Phosphoglycoprotein(MEPE).

Research Area

Cancer Biology, Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MEPE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MEPE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MEPE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MEPE in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.139 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

OF45 ELISA Kit, Asarm Motif-Containing ELISA Kit, Osteoblast/Osteocyte Factor 45 ELISA Kit

Quick Database Links

UniProt

Q9NQ76