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Catalog Number | orb1477848 |
---|---|
Category | Proteins |
Description | Recombinant Human Melanoma-associated antigen 4(MAGEA4) (Active) |
Tag | C-terminal 10xHis-tagged |
Form/Appearance | Lyophilized powder |
Purity | Greater than 90% as determined by SDS-PAGE. |
MW | 36.7 kDa |
UniProt ID | P43358 |
Protein Sequence | MSSEQKSQHCKPEEGVEAQEEALGLVGAQAPTTEEQEAAVSSSSPLVPGTLEEVPAAESAGPPQSPQGASALPTTISFTCWRQPNEGSSSQEEEGPSTSPDAESLFREALSNKVDELAHFLLRKYRAKELVTKAEMLERVIKNYKRCFPVIFGKASESLKMIFGIDVKEVDPASNTYTLVTCLGLSYDGLLGNNQIFPKTGLLIIVLGTIAMEGDSASEEEIWEELGVMGVYDGREHTVYGEPRKLLTQDWVQENYLEYRQVPGSNPARYEFLWGPRALAETSYVKVLEHVVRVNARVRIAYPSLREAALLEEEEGV |
Protein Length | Full Length |
Source | Mammalian cell |
Expression System | Expression Region: 1-317aa. Protein Length: Full Length |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human MAGEA4 at 2 μg/Ml can bind anti-MAGEA4 recombinant antibody, the EC50 is 30.33-43.10 ng/mL. |
Endotoxins | Less than 1.0 EU/ug as determined by LAL method. |
Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 |
Alternative names | (Cancer/testis antigen 1.4) (CT1.4) (MAGE-4 antige Read more... |
Note | For research use only |
Application notes | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Expiration Date | 6 months from date of receipt. |
Unconjugated | |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 35.7 kDa after removal of the signal peptide. The apparent molecular mass of MAGEA4-His is approximately 35-55 kDa due to glycosylation. | |
Mammalian |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
34.8 kDa | |
E.Coli |
ELISA, WB | |
Greater than 95% as determined by SDS-PAGE | |
13.5 kDa | |
E.Coli |
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