Human Macrophage Inflammatory Protein 5 (MIP5) ELISA Kit

SKU: orb778438

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 5(MIP5).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIP5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.1 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CCL15 ELISA Kit, SCYA15 ELISA Kit, HCC-2 ELISA Kit, NCC-3 ELISA Kit, SCYL3 ELISA Kit, Lkn-1 ELISA Kit, MIP1-D ELISA Kit, MIP1d ELISA Kit, MIP-1d ELISA Kit, HMRP-2B ELISA Kit, Leukotactin-1 ELISA Kit, Chemokine C-C-Motif Ligand 15 ELISA Kit, MIP-1 Delta ELISA Kit, Small-inducible cytokine A15 ELISA Kit

Quick Database Links

UniProt

Q16663