Human Macrophage Inflammatory Protein 3 Alpha (MIP3a) ELISA Kit

SKU: orb774984

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha(MIP3a).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 500 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIP3a in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 7.82-500 pg/mL
• Sensitivity: 2.9 pg/mL
• Concentration: 500 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MIP3 Alpha, CCL20 ELISA Kit, CKb4 ELISA Kit, LARC ELISA Kit, MIP3-A ELISA Kit, SCYA20 ELISA Kit, ST38 ELISA Kit, Small Inducible Cytokine Subfamily A(Cys-Cys)Member 20 ELISA Kit, Beta-Chemokine Exodus-1 ELISA Kit, Liver And Activation-Regulated Chemokine ELISA Kit

Quick Database Links

UniProt

P78556

References

Rui Yuan 1, Qi-Chao Sun 2, Yue-Peng An 1, Qing Zhang 1, Qiong Zhang 1, Yue Li 3 Eriodictyol inhibits the proliferation and inflammatory response in keratinocytes in psoriasis through inactivating DYRK1A-mediated endoplasmic reticulum stress J Asian Nat Prod Res, (2025)

PubMed: 39987551

Applications

ELISA

Reactivity

Human