Human Macrophage Inflammatory Protein 1 Beta (MIP1b) ELISA Kit

SKU: orb774982

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 1 Beta(MIP1b).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MIP1b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MIP1b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MIP1b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MIP1b in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.2 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MIP1 Beta,CCL4 ELISA Kit, ACT2 ELISA Kit, G-26 ELISA Kit, LAG1 ELISA Kit, MIP1-B ELISA Kit, SCYA4 ELISA Kit, Chemokine C-C-Motif Ligand 4 ELISA Kit, Small Inducible Cytokine A4 ELISA Kit, Homologous To Mouse Mip-1b ELISA Kit

Quick Database Links

UniProt

P13236

References

A Gąsiorowska, M Romanowski Efficacy and Safety of a Mixture of Microencapsulated Sodium Butyrate, Probiotics, and Short Chain Fructooligosaccharides in Patients with Irritable Bowel Syndrome—A Randomized, Double-Blind, Placebo-Controlled Study Journal of Clinical Medicine, (2024)

PubMed:

Applications

ELISA

Reactivity

Human