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Catalog Number | orb257372 |
---|---|
Category | Proteins |
Description | Human M-CSF R Protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2). |
Reactivity | Human |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 80.6 kDa |
Target | M-CSF R, CSF1R, CD115 |
UniProt ID | P07333 |
Source | Human M-CSF R Protein, Fc Tag (orb257372) is expressed from human 293 cells (HEK293). It contains AA Ile 20 - Glu 512 (Accession # NP_005202.2). |
Expression System | HEK293 |
Biological Origin | Human |
Biological Activity | Human M-CSF Protein, His Tag captured on CM5 Chip via anti-His antibody can bind Human M-CSF R Protein, Fc Tag with an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested). |
Expression Region | Ile 20 - Glu 512 |
Endotoxins | 0.1 EU per μg |
NCBI | NP_005202.2 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | CSF1R,C-FMS,CD115,CSFR,FIM2,FMS,M-CSFR Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 80.6 kDa. The protein migrates as 100-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Human M-CSF R protein
Human M-CSF Protein, His Tag (Cat. No. orb402822) captured on CM5 Chip via anti-His antibody can bind Human M-CSF R Protein, Fc Tag (Cat. No. orb257372) with an affinity constant of 1.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).
Unconjugated | |
95% | |
81.9 kDa | |
Cynomolgus M-CSF R, Fc Tag (orb18114) is expressed from human 293 cells (HEK293). It contains AA Ile 99 - Pro 596 (Accession # G7P8P1). |
Unconjugated | |
95% | |
81.8 kDa | |
Mouse M-CSF R, Fc Tag (orb383508) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Ser 511 (Accession # NP_001032948). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 37.2 kDa after removal of the signal peptide. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 38.8 kDa after removal of the signal peptide. The apparent molecular mass of CSF1R(291-401)-hFc is approximately 35-55 kDa due to glycosylation. |