Human Lysosomal Associated Membrane Protein 2 (LAMP2) ELISA Kit

SKU: orb775856

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysosomal Associated Membrane Protein 2(LAMP2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAMP2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 62.5-4000 pg/mL
• Sensitivity: 26.1 pg/mL
• Concentration: 4000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD107-B ELISA Kit, CD107b ELISA Kit, LAMP-2C ELISA Kit, LAMPB ELISA Kit, LGP96 ELISA Kit, Lysosome-associated membrane glycoprotein 2 ELISA Kit, CD107 antigen-like family member B ELISA Kit

Quick Database Links

UniProt

P13473