Human Lymphocyte Function Associated Antigen 3 (LFA3) ELISA Kit

SKU: orb2667844

Description

Human Lymphocyte Function Associated Antigen 3 (LFA3) ELISA Kit

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology

Images & Validation

• Application Notes:
  standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LFA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LFA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LFA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LFA3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, cell lysatesand other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 31.25-2000 pg/mL
• Sensitivity: 12.8 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD58; Ag3; Surface glycoprotein LFA-3

Quick Database Links

UniProt

P19256