Human LMO7(LIM Domain Only Protein 7) Microsample ELISA Kit

SKU: orb2997576

Description

Human LMO7(LIM Domain Only Protein 7) Microsample ELISA Kit

Images & Validation

• Application Notes:
  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LMO7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LMO7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LMO7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LMO7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Key Properties

• Sample Types: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.054 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

FBX20; FBXO20; LOMP; F-Box Only Protein 20

Quick Database Links

UniProt

Q8WWI1