Human Liver Receptor Homolog 1 (LRH1) ELISA Kit

SKU: orb778302

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Liver Receptor Homolog 1(LRH1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LRH1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LRH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LRH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LRH1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.115 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NR5A2 ELISA Kit, B1F ELISA Kit, B1F2 ELISA Kit, CPF ELISA Kit, FTF ELISA Kit, FTZ-F1 ELISA Kit, LRH-1 ELISA Kit, hB1F ELISA Kit, hB1F-2 ELISA Kit, Hepatocytic transcription factor ELISA Kit, Alpha-1-fetoprotein transcription factor ELISA Kit, Nuclear Receptor Subfamily 5,Group A,Member 2 ELISA Kit

Quick Database Links

UniProt

O00482