Human Lipocalin 1 (LCN1) ELISA Kit

SKU: orb775640

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipocalin 1(LCN1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LCN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LCN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LCN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LCN1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, saliva, tears and other biological fluids and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.63 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

TP ELISA Kit, PMFA ELISA Kit, VEGP ELISA Kit, Tlc ELISA Kit, Tear Prealbumin ELISA Kit, Tear lipocalin ELISA Kit, Protein Migrating Faster Than Albumin ELISA Kit, Von Ebner Gland Protein ELISA Kit

Quick Database Links

UniProt

P31025