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| Catalog Number | orb775323 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Lipoprotein(LIPD). |
| Reactivity | Human |
| Concentration | 40 ng/mL |
| UniProt ID | P06858 |
| Tested applications | ELISA |
| Application notes | standard: 40 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LIPD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIPD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LIPD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LIPD in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 0.63-40 ng/mL |
| Sensitivity | 0.235 ng/mL |
| Sample Types | serum, plasma, tissue homogenates, cell lysates and other biological fluids |
| Alternative names | LPL ELISA Kit, Lipoprotein Lipase ELISA Kit |
| Note | For research use only |
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Human LPL ELISA Kit [orb407526]
Human
31.25 pg/mL-2000 pg/mL
7.81 pg/mL
10 x 96 T, 5 x 96 T, 48 T, 96 T, 24 T
