Human Lipase, Bile Salt Dependent (BSDL) ELISA Kit

SKU: orb777467

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase, Bile Salt Dependent(BSDL).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BSDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BSDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BSDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BSDL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.057 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CEL ELISA Kit, FAP ELISA Kit, BAL ELISA Kit, BSSL ELISA Kit, FAPP ELISA Kit, LIPA ELISA Kit, MODY8 ELISA Kit, Carboxyl Ester Lipase ELISA Kit, Bile salt-stimulated lipase ELISA Kit, Bucelipase ELISA Kit, Cholesterol esterase ELISA Kit, Pancreatic lysophospholipase ELISA Kit, Sterol esterase ELISA Kit

Quick Database Links

UniProt

P19835