Human Leucyl/Cystinyl Aminopeptidase (LNPEP) ELISA Kit

SKU: orb777688

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucyl/Cystinyl Aminopeptidase(LNPEP).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LNPEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LNPEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LNPEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LNPEP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.062 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CAP ELISA Kit, PLAP ELISA Kit, OTASE ELISA Kit, IRAP ELISA Kit, Oxytocinase ELISA Kit, Insulin-regulated membrane aminopeptidase ELISA Kit, Insulin-responsive aminopeptidase ELISA Kit, Placental leucine aminopeptidase ELISA Kit

Quick Database Links

UniProt

Q9UIQ6