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| Catalog Number | orb780990 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 4B(LRRC4B). |
| Reactivity | Human |
| Concentration | 20 ng/mL |
| UniProt ID | Q9NT99 |
| Tested applications | ELISA |
| Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LRRC4B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LRRC4B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LRRC4B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LRRC4B in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Type | Sandwich |
| Assay Time | 3.5h |
| Range | 0.32-20 ng/mL |
| Sensitivity | 0.127 ng/mL |
| Sample Types | Tissue homogenates and other biological fluids. |
| Alternative names | HSM ELISA Kit, LRIG4 ELISA Kit, NGL-3 ELISA Kit, N Read more... |
| Note | For research use only |
