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Description
Images & Validation
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Key Properties
−| Expression System | Human cell |
|---|---|
| Biological Activity | ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 4 Heterodimer (C-Flag, C-6His)(Cat#orb2992902) at 10μg/ml (100 μl/well) can bind Recombinant Human Laminin521 E8 Protein(Cat#orb3002520).The ED50 of Recombinant Human Laminin521 E8 Protein(Cat#orb3002520) is 31.75 ng/ml(Regularly tested).. Cell Based Assay: Measured by its ability to support iPS cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 94 ng/mL.（Regularly tested） |
| Tag | N-His & N-Flag & N-HA |
| Expression Region | Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609 |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
| MW | Predicted: 86.7&25.8&28.5 KDa. Observed: (28-32)&(38-48)&(100-120) KDa, reducing conditions |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM PB，2% Sucrose，4% Mannitol，0.02%Tween-80，pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Laminin 521; LN521
Quality Guarantee
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Protocol Information
Protein Handling and Storage Guide
Protein Handling Guide
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