Human Laminin 521

• Catalog Number: orb3002520
• Category: Proteins
• Description: Recombinant Human Laminin521 is produced by our Mammalian expression system and the target gene encoding Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609 is expressed with a His, Flag and HA tag at N terminus respectively.
• Tag: N-His & N-Flag & N-HA
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of 20mM PB，2% Sucrose，4% Mannitol，0.02%Tween-80，pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• UniProt ID: O15230, P11047, P55268
• MW: Predicted: 86.7&25.8&28.5 KDa. Observed: (28-32)&(38-48)&(100-120) KDa, reducing conditions
• Expression System: Human cell
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• Biological Activity: ELISA: Immobilized Recombinant Human Integrin alpha 6 beta 4 Heterodimer (C-Flag, C-6His)(Cat#orb2992902) at 10μg/ml (100 μl/well) can bind Recombinant Human Laminin521 E8 Protein(Cat#orb3002520).The ED50 of Recombinant Human Laminin521 E8 Protein(Cat#orb3002520) is 31.75 ng/ml(Regularly tested).. Cell Based Assay: Measured by its ability to support iPS cell attachment and spreading when used as a substratum for cell culture. The ED50 for this effect is 94 ng/mL.（Regularly tested）
• Expression Region: Ala2534-Ala3322&Leu1573-Gln1798&Asn1364-Pro1609
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Laminin 521; LN521
• Note: For research use only