Human Laminin 221 E8 Protein

• Catalog Number: orb3002523
• Category: Proteins
• Description: Recombinant Human Laminin 221 is produced by our Mammalian expression system and the target gene encoding Leu1900-Ala2722&Leu1573-Gln1798&Asn1364-Pro1609 is expressed with a 6His, Flag and HA tag at N terminus respectively.
• Tag: N-His &N-Flag&N-HA
• Form/Appearance: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
• UniProt ID: P24043, P11047, P55268
• MW: Predicted: 91.8&25.8&28.5 KDa. Observed: (27-31)&(35-45)&(110-145)
• Expression System: Human Cells
• Endotoxins: < 0.01 EU/µg as determined by LAL test.
• Biological Activity: ELISA: Immobilized Recombinant Human Integrin alpha7X2B beta 1 at 5μg/ml (100 μl/well) can bind Recombinant Human Laminin 221 E8 Protein (Cat#orb3002523) *.*: Biotinylated by NHS-biotin prior to testing. The ED50 of Recombinant Human Laminin 221 E8 Protein (Cat#orb3002523) is 6.06 ng/ml. (Regularly tested)
• Expression Region: Leu1900-Ala2722&Leu1573-Gln1798&Asn1364-Pro1609
• Storage: Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
• Alternative names: Laminin 221; LN221
• Note: For research use only