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Catalog Number | orb1291334 |
---|---|
Category | Assays and Kits |
Description | Human L-Carnitine ELISA Kit |
Reactivity | Human |
Concentration | 20 ng/mL |
Tested applications | ELISA |
Application notes | standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human L-Carnitine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human L-Carnitine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human L-Carnitine in the samples is then determined by comparing the OD of the samples to the standard curve |
Assay Time | 3.5h |
Range | 0.32-20 ng/mL |
Sensitivity | 0.119 ng/mL |
Sample Types | serum, plasma, urine, tissue homogenates |
Alternative names | L-Carnitine |
Note | For research use only |
Human | |
15.625-1000pg/ml | |
9.375pg/ml |
Human | |
0.312 ng/mL-20 ng/mL | |
0.078 ng/mL |
Human | |
0.156-10ng/mL | |
0.085ng/mL |