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Human L-Carnitine ELISA Kit

Catalog Number: orb1291334

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1291334
CategoryAssays and Kits
DescriptionHuman L-Carnitine ELISA Kit
ReactivityHuman
Concentration20 ng/mL
Tested applicationsELISA
Application notesstandard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human L-Carnitine. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human L-Carnitine. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human L-Carnitine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human L-Carnitine in the samples is then determined by comparing the OD of the samples to the standard curve
Assay Time3.5h
Range0.32-20 ng/mL
Sensitivity0.119 ng/mL
Sample Typesserum, plasma, urine, tissue homogenates
Alternative namesL-Carnitine
NoteFor research use only
Human L-Carnitine ELISA Kit