Human KRAS Protein, His Tag

SKU: orb1173815

Description

Recombinant human KRAS protein with C-terminal 6×His tag

Research Area

Cancer, Cardiovascular Disease, Epigenetics

Key Properties

• Source: Mammalian
• Expression System: HEK293
• Target: KRAS
• Reactivity: Human
• Tag: C-6×His Tag
• Molecular Weight: The protein has a predicted molecular mass of 21.8 kDa after removal of the signal peptide. The apparent molecular mass of KRAS-His is approximately 15-25 kDa due to glycosylation.
• Expression Region: KRAS(Thr2-Cys185) 6×His tag
• Purification: Purified from cell culture supernatant by affinity chromatography
• Purity: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
• Form/Appearance: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Buffer/Preservatives: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
• Reconstitution: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

C-K-RAS;c-Ki-ras2;CFC2;K-Ras;K-RAS2A;K-RAS2B;K-RAS4A;K-RAS4B;KI-RAS;KRAS1;KRAS2;NS;NS3;RALD;RASK2

Human B7-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human B7-1, mFc-His tagged protein (orb757420) can bind anti-B7-1 monoclonal antibody, Rabbit mAb clone: DM111 in a linear range of 0.13-80 ng/ml.

Flow cytometry analysis with 15 μg/mL Human B7-1 Protein, mFc-His tag (orb757420) on Expi293 cells transfected with human CD28 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Quick Database Links

Gene Symbol

KRAS

UniProt

P01116