Human Kinesin Family, Member 5A (KIF5A) ELISA Kit

• Catalog Number: orb777794
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 5A(KIF5A).
• Reactivity: Human
• Concentration: 20 ng/mL
• UniProt ID: Q12840
• Tested applications: ELISA
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KIF5A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KIF5A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KIF5A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KIF5A in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.114 ng/mL
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Alternative names: NKHC ELISA Kit, MY050 ELISA Kit, SPG10 ELISA Kit,
• Note: For research use only