Human Kinesin Family, Member 20A (KIF20A) ELISA Kit

SKU: orb777548

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 20A(KIF20A).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KIF20A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KIF20A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KIF20A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KIF20A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.048 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

MKLP2 ELISA Kit, RAB6KIFL ELISA Kit, GG10_2 ELISA Kit, RAB6 Interacting,Kinesin-Like ELISA Kit, Rab Kinesin 6 ELISA Kit, Mitotic kinesin-like protein 2 ELISA Kit, Rabkinesin-6 ELISA Kit

Quick Database Links

UniProt

O95235