Human Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) ELISA Kit

SKU: orb778221

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Killer Cell Immunoglobulin Like Receptor 2DS4(KIR2DS4).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KIR2DS4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KIR2DS4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KIR2DS4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KIR2DS4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 1.57-100 ng/mL
• Sensitivity: 0.65 ng/mL
• Concentration: 100 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CD158I ELISA Kit, KIR1D ELISA Kit, KIR412 ELISA Kit, KKA3 ELISA Kit, NKAT8 ELISA Kit, PAX ELISA Kit, Cl-39 ELISA Kit, Natural killer-associated transcript 8 ELISA Kit, CD158 antigen-like family member I ELISA Kit, NK receptor CL-39/CL-17 ELISA Kit

Quick Database Links

UniProt

P43632