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| Catalog Number | orb777943 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 6A(KRT6A). |
| Reactivity | Human |
| Concentration | 1000 pg/mL |
| UniProt ID | P02538 |
| Tested applications | ELISA |
| Application notes | standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT6A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT6A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT6A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT6A in the samples is then determined by comparing the OD of the samples to the standard curve |
| Assay Time | 3.5h |
| Range | 15.63-1000 pg/mL |
| Sensitivity | 6.6 pg/mL |
| Sample Types | Serum, plasma, tissue homogenates and other biological fluids |
| Alternative names | K6A ELISA Kit, KRT6C ELISA Kit, KRT6D ELISA Kit, C Read more... |
| Note | For research use only |
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Human KRT6A ELISA Kit [orb404689]
Human
0.156 ng/mL-10 ng/mL
0.039 ng/mL
24 T, 10 x 96 T, 5 x 96 T, 48 T, 96 T
