Human Keratin 5 (KRT5) ELISA Kit

SKU: orb777885

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 5(KRT5).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KRT5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KRT5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KRT5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KRT5 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 15.63-1000 pg/mL
• Sensitivity: 6.8 pg/mL
• Concentration: 1000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

CK5 ELISA Kit, DDD ELISA Kit, EBS2 ELISA Kit, K5 ELISA Kit, KRT5A ELISA Kit, KRT5-A ELISA Kit, Cytokeratin 5 ELISA Kit, 58 kDa cytokeratin ELISA Kit, Type-II keratin Kb5 ELISA Kit, Epidermolysis Bullosa Simplex Dowling-Meara/Kobner/Weber-Cockayne Types ELISA Kit

Quick Database Links

UniProt

P13647