Human Karyopherin Beta (KPNb1) ELISA Kit

SKU: orb778916

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Karyopherin Beta(KPNb1).

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 50 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KPNb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KPNb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KPNb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KPNb1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.79-50 ng/mL
• Sensitivity: 0.32 ng/mL
• Concentration: 50 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

IMB1 ELISA Kit, IPOB ELISA Kit, Impnb ELISA Kit, Impnb ELISA Kit, IPO1 ELISA Kit, NTF97 ELISA Kit, PTAC97 ELISA Kit, Importin subunit beta-1 ELISA Kit, Importin-90 ELISA Kit, Nuclear factor p97 ELISA Kit, Pore targeting complex 97 kDa subunit ELISA Kit

Quick Database Links

UniProt

Q14974