Human Kallikrein 8 (KLK8) ELISA Kit

SKU: orb775715

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 8(KLK8) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 8(KLK8).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 50000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK8 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK8 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 781.25-50000 pg/mL
• Sensitivity: 216.4 pg/mL
• Concentration: 50000 pg/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

NP ELISA Kit, HNP ELISA Kit, NRPN ELISA Kit, PRSS19 ELISA Kit, TADG14 ELISA Kit, BSP1 ELISA Kit, Neuropsin ELISA Kit, Ovasin ELISA Kit, Brain Serine Protease 1 ELISA Kit, Tumor Associated Differentially Expressed Gene 14 ELISA Kit, Serine protease 19 ELISA Kit

Quick Database Links

UniProt

O60259