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Human Kallikrein 10 (KLK10) ELISA Kit

Catalog Number: orb774975

DispatchUsually dispatched within 5-10 working days
$ 310.00
Catalog Numberorb774975
CategoryAssays and Kits
DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Kallikrein 10(KLK10) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Kallikrein 10(KLK10).
ReactivityHuman
Concentration10000 pg/mL
UniProt IDO43240
Tested applicationsELISA
Application notesstandard: 10000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK10 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK10 in the samples is then determined by comparing the OD of the samples to the standard curve
Assay TypeCompetitive
Assay Time2.5h
Range156.25-10000 pg/mL
Sensitivity48.8 pg/mL
Sample TypesSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Alternative namesNES1 ELISA Kit, PRSSL1 ELISA Kit, Kallikrein-Relat
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NoteFor research use only
Human Kallikrein 10 (KLK10) ELISA Kit

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