Human Junctional Adhesion Molecule 1 (JAM1) ELISA Kit

SKU: orb777786

Description

The Human Junctional Adhesion Molecule 1 (JAM1) ELISA Kit uses a sandwich enzyme immunoassay to quantify Junctional Adhesion Molecule 1 (JAM1) in human serum, plasma, tissue homogenates and other biological fluids. The kit has a detection range of 0.32-20 ng/mL and a sensitivity of 0.133 ng/mL.

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human JAM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human JAM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human JAM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human JAM1 in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: Human
• Sample Types: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.133 ng/mL
• Concentration: 20 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

CD321 ELISA Kit, F11R ELISA Kit, KAT ELISA Kit, JAM-A ELISA Kit, JAMA ELISA Kit, JCAM ELISA Kit, PAM1 ELISA Kit, Coagulation Factor XI Receptor ELISA Kit, Platelet F11 receptor ELISA Kit, Platelet adhesion molecule 1 ELISA Kit

Quick Database Links

UniProt

Q9Y624