Human Interleukin 40 (IL-40) ELISA Kit

• Catalog Number: orb1496474
• Category: Assays and Kits
• Description: Human IL-40 ELISA Kit
• Reactivity: Human
• Concentration: 20 ng/mL
• UniProt ID: Q6UX52
• Application notes: standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-40 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.32-20 ng/mL
• Sensitivity: 0.13 ng/mL
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Alternative names: IL-40; UNQ464
• Note: For research use only